|
KMID : 0829320110140030103
|
|
Korean Journal of Clinical Microbiology
2011 Volume.14 No. 3 p.103 ~ p.109
|
|
|
Evaluation of MolecuTech Real MTB-ID for MTB/NTM Detection Using Direct Specimens
|
|
Wang Hye-Young
Jin Hyun-Woo
Bang Hye-Eun
Choi Yeon-Im
Lee Hye-Young
Park Eun-Mi
Koh Won-Jung
|
|
|
Abstract
|
|
|
Background: The differentiation of Mycobacterium tuberculosis MTB) from nontuberculous mycobacteria (NTM) is of primary importance for infection control and choice of antimicrobial therapy. The diagnosis of diseases caused by NTM is difficult because NTM are prevalent in the environment and have fastidious properties. In this study, we evaluated the0 real-time PCR-based MTB/NTM detection kit for its usefulness in discrimination of MTB and NTM species.
Methods: A total of 155 sputum specimens whose AFB staining smear and culture were positive were used for this study. Among them, 59 and 96 samples had been identified as MTB and NTM, respectively. DNA obtained from sputum specimens was subjected to analysis with MolecuTech Real MTB-IDⓡ (M&D, Korea) real-time PCR-based MTB/NTM detection kit. Subsequently, the results of MolecuTech Real MTBIDⓡ were compared with AFB staining smear and culture results.
Results: The positive rate of MolecuTech Real MTBIDⓡ to detect MTB and NTM was 98.3% (58/59) and 97.9 (94/96), respectively, using sputum specimens.
Conclusion: For detection of MTB/NTM, the sensitivity and specificity of MolecuTech Real MTB-IDⓡ were comparable to those of conventional methods. Therefore, this study suggests the usefulness of real-time PCR-based MolecuTech Real MTB-IDⓡ for rapid detection of MTB/NTM from direct specimens.
|
|
|
KEYWORD
|
|
|
Mycobacterium tuberculosis, Non-tuberculous mycobacteria, Real-time PCR
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|